Genomic Dna Purification
ثبت نشده
چکیده
The Extract-N-AmpTM Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by PCR (Fig. 1). This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR Ready mix, especially formulated for amplification directly from the extract. This formulation uses an antibody based Hot Start, for specific amplification. The PCR master mix comes in two formulations: Extract-N-AmpTM Blood PCR mix and REDExtract-N-AmpTM Blood PCR mix. The REDExtract-N-AmpTM Blood PCR kit contains a tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.
منابع مشابه
An efficient and simple CTAB based method for total genomic DNA isolation from low amounts of aquatic plants leaves with a high level of secondary metabolites
An efficient DNA isolation protocol specifically modified to get pure quality DNA required for molecular studieshas been reported in this paper. Some aquatic plants (Potamogeton spp., Ceratophyllum demersum and Myriophyllum spicatum) were used for the study. The protocol developed will be useful in getting high and pure DNA. Instead of using the available DNA extraction kits, this protocol can ...
متن کاملAn efficient purification and fractionation of genomic DNA from soil by modified troughing method.
AIMS The aim of this study was to utilize a modified troughing method for purification of large genomic DNA obtained from microbiota in natural environment and for fractionation of genomic DNA into many size ranges that facilitates construction of metagenomic library. METHODS AND RESULTS Genomic DNA extracted from soil or termite gut was purified by the modified troughing method which utilize...
متن کاملEvaluation of methods for the extraction and purification of DNA of cultured Lactobacillus colony isolated from dairy products
Article Type Full Length Research Article Lactic acid bacteria are very significant to human health due to the production of some antimicrobial substances and ability to inhibit pathogenic bacteria. Furthermore, the bacteria are also used as starter culture in the production of various foods. The aim of this study was to evaluate five methods for the extraction and purification of DNA from 6 is...
متن کاملPhenol-stacked carbon nanotubes: A new approach to genomic DNA isolation from plants
Extraction of intact quality DNA from plant tissues, especially those rich in secondary metabolites, is often challenging. Literally, hundreds of different DNA isolation protocols from various plant species have been published over the last decades. Although many commercial DNA isolation kits are convenient and designed to be safe, their cost and availability cause limitations in small molecula...
متن کاملGenomic Dna Extraction from Dried Blood Spots
We have developed an efficient and simple method for extracting and purifying genomic DNA from dried blood stored on filter paper. The quality of the genomic DNA extracted is tested by PCR amplification of a 255-bp fragment of the PAX8 gene sequence and the PCR products are determined for further genetic studies by single strand conformation polymorphism (SSCP) analysis. Larger DNA sequences of...
متن کاملRapid DNA Extraction Protocol from Stool, Suitable for Molecular Genetic Diagnosis of Colon Cancer
Colorectal cancer (CRC) is one of the most common forms of cancers in the world and is curable if diagnosed at the early stage. Analysis of DNA extracted from stool specimens is a recent advantage to cancer diagnostics. Many protocols have been recommended for DNA extraction from stool, and almost allof them are difficult and time consuming, dealing with high amount of toxic materials like phen...
متن کامل